You are not showing your markup, but remember that any button click, control with automatic postback, or any event firing on the page will re-run the page load event.

So, in theory, even a simple button click might rerun the code that loads the combo box. So every event, every click may add or load the combobox again.

Therefore, the design pattern of almost every page is to load grids, list boxes, drop-down boxes, etc. only once.

So your code should look like this:

Protected Sub Page_Load(ByVal sender As Object, ByVal e As System.EventArgs) Handles Me.Load If Not IsPostBack Then LoadTable() End If End Sub Public Sub LoadTable() Using Connection As New MySqlConnection("server=localhost; userid=root; database=setting;") Using Command As New MySqlCommand("SELECT * from table", Connection) Connection.Open Dim MyTable As New DataTable MyTable.Load(Command.ExecuteReader) Ddlname.DataSource = DataTable Ddlname.DataTextField = "Name" Ddlname.DataValueField = "Name" Ddlname.DataBind() End Using End Using End Sub

So make sure your page loading code has the very important If Not IsPostBack so that you can actually only load the code that loads the combobox once.

So this "is a postback" test? 99% of your pages will work this way. I often think that asp.net pages should have a "firstLoad" event because it fires every time the page loads, and this is true for any buttons and any code that triggers page postbacks. Therefore, your combobox will load (and grow) again and again because you run the code that loads the grid, listbox, or dropdown every time you load the page. So adopt, use and "like" the IsPostBack test - you do it for all your pages, and 99% or more of them require this test.

In fact, if you don't apply the above suggestions, you will almost never be able to build any functional web page.